phospho perk  (Cell Signaling Technology Inc)

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: DOCK10 Regulates Insulin Hypersecretion in Insulinoma and Serves as a Diagnostic and Therapeutic Target

doi: 10.1016/j.jcmgh.2025.101705

Figure Lengend Snippet: DOCK10 regulates insulin secretion via CDC42 in MIN6 cells. ( A ) Dock10 mRNA levels by qPCR in control and DOCK10-knockdown (KD) MIN6 cells. ( B ) ELISA measurement of insulin secretion after stimulation with 3 mM glucose, 25 mM glucose, or 3 mM glucose + 30 mM KCl. ( C ) ELISA measurement of intracellular insulin levels in control and knockdown MIN6 cells (n = 3/group). ( D ) Schematic of Dock10–Cdc42 pathway regulating GSIS; ML141, a Cdc42 inhibitor. ( E ) Protein levels of phosphorylated PAK1 (p-PAK1), total PAK1, and β-actin after glucose stimulation (2-hour fasting) in control and knockdown cells by Simple Western; table shows p-PAK1/PAK1 ratio normalized to β-actin. ( F ) Protein levels after 1-hour fasting and ML141 or vehicle (DMSO) pretreatment, assessed by Simple Western; table shows normalized p-PAK1/PAK1 ratios. ( G ) ELISA measurement of insulin secretion after ML141 or vehicle pretreatment under the same stimulations (n = 3/group). Data were pooled from 3 independent experiments for panels in ( A–C ) and ( G ). Values are presented as mean ± SD. Statistical analysis was performed using unpaired Student’s t -test. ns, not significant; ∗∗ P < .01; ∗∗∗ P < .005.

Article Snippet: phospho PAK1 , Cell Signaling Technology , 2601S , 1:10.

Techniques: Control, Knockdown, Enzyme-linked Immunosorbent Assay, Simple Western